Difference between revisions of "Part:BBa K4220008"

(→‎Introduction)
 
(15 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.
 
The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.
  
−
==P2Y2==
+
*P2Y2
 +
**In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]
  
−
In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]
+
*P2A
 +
**After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.
  
−
==P2A==
+
*mCherry
−
 
+
**To report the successful cleavage of P2A peptide.
−
After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.
+
−
 
+
−
==mCherry==
+
−
 
+
−
To report the successful cleavage of P2A peptide.
+
  
 
==Results==
 
==Results==
Line 23: Line 20:
 
1.The P2A peptide can work effectively:
 
1.The P2A peptide can work effectively:
  
−
[[File:p2a.jpg|550px|options|caption]]
+
[[File:neu81.jpg|550px|options|caption]]
 +
 
  
 
2.The production of P2Y2:
 
2.The production of P2Y2:
  
−
[[File:p2y2(mcherry).jpg|550px|options|caption]]
+
[[File:neu82.jpg|550px|options|caption]]
 +
 
 +
==References==
 +
1 Scott, B. M. et al. Self-tunable engineered yeast probiotics for the treatment of inflammatory bowel disease. Nature Medicine 27, 1212-1222, doi:10.1038/s41591-021-01390-x (2021).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:11, 1 October 2022


P2Y2-P2A-mCherry

Introduction

The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.

  • P2Y2
    • In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]
  • P2A
    • After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.
  • mCherry
    • To report the successful cleavage of P2A peptide.

Results

1.The P2A peptide can work effectively:

caption


2.The production of P2Y2:

caption

References

1 Scott, B. M. et al. Self-tunable engineered yeast probiotics for the treatment of inflammatory bowel disease. Nature Medicine 27, 1212-1222, doi:10.1038/s41591-021-01390-x (2021).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1095
    Illegal AgeI site found at 389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 570
    Illegal SapI site found at 615