Difference between revisions of "Part:BBa K4220100"
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4220100 short</partinfo> | <partinfo>BBa_K4220100 short</partinfo> | ||
+ | |||
+ | ==Introduction== | ||
This is a kill switch in yeast which can let the yeast survive only in the presence of copper ions. | This is a kill switch in yeast which can let the yeast survive only in the presence of copper ions. | ||
+ | |||
+ | ==How to kill the yeast? == | ||
+ | |||
+ | HSF1: essential gene in yeast [1] | ||
+ | |||
+ | ==How to determine when to kill? == | ||
+ | |||
+ | Copper ion: abundant in the intestine; scarce in the environment [2] | ||
+ | |||
+ | ==Others== | ||
+ | |||
+ | HIS3-NatR-ADH1: resistant gene expression for screening | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 12: | Line 26: | ||
<partinfo>BBa_K4220100 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4220100 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | ==References== | ||
+ | |||
+ | 1 Kopczynski, J. B., Raff, A. C. & Bonner, J. J. Translational readthrough at nonsense mutations in the HSF1 gene of Saccharomyces cerevisiae. Mol Gen Genet 234, 369-378, doi:10.1007/BF00538696 (1992). | ||
+ | |||
+ | 2 Nose, Y., Kim, B. E. & Thiele, D. J. Ctr1 drives intestinal copper absorption and is essential for growth, iron metabolism, and neonatal cardiac function. Cell metabolism 4, 235-244, doi:10.1016/j.cmet.2006.08.009 (2006). | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 07:00, 1 October 2022
HSF1up-HIS3+NatR+ADH1+CUP1-HSF ORF
Introduction
This is a kill switch in yeast which can let the yeast survive only in the presence of copper ions.
How to kill the yeast?
HSF1: essential gene in yeast [1]
How to determine when to kill?
Copper ion: abundant in the intestine; scarce in the environment [2]
Others
HIS3-NatR-ADH1: resistant gene expression for screening
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 247
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 367
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 512
References
1 Kopczynski, J. B., Raff, A. C. & Bonner, J. J. Translational readthrough at nonsense mutations in the HSF1 gene of Saccharomyces cerevisiae. Mol Gen Genet 234, 369-378, doi:10.1007/BF00538696 (1992).
2 Nose, Y., Kim, B. E. & Thiele, D. J. Ctr1 drives intestinal copper absorption and is essential for growth, iron metabolism, and neonatal cardiac function. Cell metabolism 4, 235-244, doi:10.1016/j.cmet.2006.08.009 (2006).