Difference between revisions of "Part:BBa K200005"
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| − | Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsa1</cite>, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.<br><br> | + | [en.wikipedia.org/wiki/Trehalose Trehalose] is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsa1</cite>, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.<br><br> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Transcription of OtsA gene is activated by osmotic stress in E. coli.<cite>2</cite> | ||
| + | mRNA of OtsA is more stable at 16°C, therefore, it is a cold inducible mRNA.<cite>1</cite> | ||
| + | OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. <cite>2</cite> | ||
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===References=== | ===References=== | ||
| − | <biblio>#otsa1 pmid=12105274 </biblio> | + | <biblio> |
| + | #otsa1 pmid=12105274 | ||
| + | #otsa2 pmid=1310094 | ||
| + | </biblio> | ||
Revision as of 16:46, 2 September 2009
OtsA: Part 1 of 2 for trehalose producing enzymes.
Sequence codes for trehalose-6-phosphate synthase enzyme. This enzyme is the first of two required in the conversion of glucose to trehalose.
This enzyme catalyses the following reaction:
UDP-glucose + D-glucose 6-phosphate -> UDP + alpha,alpha-trehalose 6-phosphate
[en.wikipedia.org/wiki/Trehalose Trehalose] is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsa1, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.
Usage and Biology
Transcription of OtsA gene is activated by osmotic stress in E. coli.2
mRNA of OtsA is more stable at 16°C, therefore, it is a cold inducible mRNA.1
OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. 2
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 383
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
- otsa1 pmid=12105274
- otsa2 pmid=1310094
</biblio>