Difference between revisions of "Part:BBa K200012"
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<partinfo>BBa_K200012 short</partinfo> | <partinfo>BBa_K200012 short</partinfo> | ||
| − | This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011). <br> | + | This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011). When it is not being repressed after 42°C induction, it acts as a strong promoter. <cite>pL2</cite> <br> |
This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051) <br> | This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051) <br> | ||
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===Requirements/Limitations=== | ===Requirements/Limitations=== | ||
*Possible crosstalk with other lambda repressors | *Possible crosstalk with other lambda repressors | ||
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<partinfo>BBa_K200012 parameters</partinfo> | <partinfo>BBa_K200012 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
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===References=== | ===References=== | ||
<biblio> | <biblio> | ||
pL1 pmid=10227165 | pL1 pmid=10227165 | ||
| + | </biblio> | ||
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| + | <biblio> | ||
| + | pL2 pmid=15652426 | ||
</biblio> | </biblio> | ||
Latest revision as of 16:38, 2 September 2009
lambda promoter (cIts responsive)
This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011). When it is not being repressed after 42°C induction, it acts as a strong promoter. pL2
This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051)
It has been shown through lysis experiments pL1 that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced.
The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature.
Requirements/Limitations
- Possible crosstalk with other lambda repressors
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio> pL1 pmid=10227165 </biblio>
<biblio> pL2 pmid=15652426 </biblio>