Difference between revisions of "Part:BBa K200012"

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This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011).  When it is not being repressed after 42°C induction, it acts as a strong promoter. <cite>pL2</cite> <br>
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This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051) <br>
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It has been shown through lysis experiments <cite>pL1</cite> that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C.  At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions.  Therefore, promoter activity is induced. <br>
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The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature. <br>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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===Requirements/Limitations===
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*Possible crosstalk with other lambda repressors
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K200012 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K200012 parameters</partinfo>
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<!-- -->
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===References===
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<biblio>
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pL1 pmid=10227165
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</biblio>
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<biblio>
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pL2 pmid=15652426
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</biblio>

Latest revision as of 16:38, 2 September 2009

lambda promoter (cIts responsive)

This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011). When it is not being repressed after 42°C induction, it acts as a strong promoter. pL2

This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051)

It has been shown through lysis experiments pL1 that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced.

The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature.


Requirements/Limitations

  • Possible crosstalk with other lambda repressors


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio> pL1 pmid=10227165 </biblio>

<biblio> pL2 pmid=15652426 </biblio>