Difference between revisions of "Part:BBa K200006"

 
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<partinfo>BBa_K200006 short</partinfo>
 
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Codes for trehalose-phosphatase enzyme. This second of two enzymes required for the conversion of glucose to trehalose. Trehalose is used to resist dessication in cells, and protect against conformational change in proteins under such conditions.
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Sequence codes for trehalose phosphatase enzyme. This enzyme is the second of two required for the conversion of glucose to trehalose. <br>
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This enzyme catalyses the following reaction: <br>
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alpha,alpha-trehalose 6-phosphate + H2O -> alpha,alpha-trehalose + phosphate  <br>
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<br>
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Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock <cite>otsb1</cite>, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.<br><br>
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Revision as of 13:20, 2 September 2009

OtsB: Part 2 of 2 coding for trehalose producing enzymes

Sequence codes for trehalose phosphatase enzyme. This enzyme is the second of two required for the conversion of glucose to trehalose.
This enzyme catalyses the following reaction:
alpha,alpha-trehalose 6-phosphate + H2O -> alpha,alpha-trehalose + phosphate

Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsb1, and is naturally produced in Escherichia Coli. We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]