Difference between revisions of "Part:BBa K200011"
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* More than one temperature sensitive mutations could lead to loss of DNA binding ability <cite>cI2</cite> | * More than one temperature sensitive mutations could lead to loss of DNA binding ability <cite>cI2</cite> | ||
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===References=== | ===References=== | ||
Revision as of 11:28, 2 September 2009
Temperature Sensitive Lamda cI Repressor
The temperature sensitive lamda cI repressor protein has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30 to 42°C, thereby allowing lambda promoter expression. cI1
The repressor normally negatively regulates the expression of genes from the bacteriophage lambda pL and pR promoters. This repressive action is strongest at 30°C. cI2
However, when the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases.
Requirements/Limitation
- More than one temperature sensitive mutations could lead to loss of DNA binding ability cI2
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio> cI1 pmid=5274442 </biblio> <biblio> cI2 pmid=10235623 </biblio>