Difference between revisions of "Part:BBa K200011"
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===Requirements===
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* More than one temperature sensitive mutations could lead to loss of DNA binding ability <cite>cI2</cite>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200011 SequenceAndFeatures</partinfo>

Revision as of 11:27, 2 September 2009

Temperature Sensitive Lamda cI Repressor

The temperature sensitive lamda cI repressor protein has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30 to 42°C, thereby allowing lambda promoter expression. cI1

The repressor normally negatively regulates the expression of genes from the bacteriophage lambda pL and pR promoters. This repressive action is strongest at 30°C. cI2

However, when the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases.


Requirements

  • More than one temperature sensitive mutations could lead to loss of DNA binding ability cI2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio> cI1 pmid=5274442 </biblio> <biblio> cI2 pmid=10235623 </biblio>