Difference between revisions of "Part:BBa K4147001:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in <i>Nicotiana tabacum</i> hairy roots with enhanced antimicrobial activity.   
+
This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in <i>Nicotiana tabacum</i> hairy roots with enhanced antimicrobial activity.
 +
 
 +
Shams, M.V., Nazarian-Firouzabadi, F., Ismaili, A. et al. Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity. Mol Biotechnol 61, 241–252 (2019). https://doi.org/10.1007/s12033-019-00153-x  
  
 
===References===
 
===References===

Revision as of 01:10, 1 October 2022


PelB-(CBD)2-DrsB1: A dermaseptin antimicrobial peptide from Phyllomedusa bicolor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
    Illegal AgeI site found at 444
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance.


Source

This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in Nicotiana tabacum hairy roots with enhanced antimicrobial activity.

Shams, M.V., Nazarian-Firouzabadi, F., Ismaili, A. et al. Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity. Mol Biotechnol 61, 241–252 (2019). https://doi.org/10.1007/s12033-019-00153-x

References