Difference between revisions of "Part:BBa K200009:Design"

(→‎References)
(→‎Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
Thermodesulfovibrio yellowstonii DSM 11347
+
Thermodesulfovibrio yellowstonii DSM 11347.
 +
 
 +
The DNA sequence was then synthesised.
  
 
===References===
 
===References===
  
 
http://www.ncbi.nlm.nih.gov/nuccore/NC_011296.1?report=fasta&from=502549&to=503325
 
http://www.ncbi.nlm.nih.gov/nuccore/NC_011296.1?report=fasta&from=502549&to=503325

Revision as of 14:03, 1 September 2009

DpnII Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.

Source

Thermodesulfovibrio yellowstonii DSM 11347.

The DNA sequence was then synthesised.

References

http://www.ncbi.nlm.nih.gov/nuccore/NC_011296.1?report=fasta&from=502549&to=503325