Difference between revisions of "Part:BBa K200009"

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As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used to cut up the genetic material within the cell, completing the cell death module.  
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As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used with TaqI to cut up the genetic material within the cell, completing the cell death module.  
 
    
 
    
  

Revision as of 12:41, 1 September 2009

DpnII Composite

DpnII is a dam sensitive restriction enzyme.
The recognition sequence of this restriction enzyme is GATC and is a 4 base cutter, leaving a 4 base overhang.


The cutting site is as shown:
NGATCN -> N / GATCN
NCTAGN -> NCTAG / N


As part of the Imperial 2009 iGEM E.ncapsulator project, DpnII was used with TaqI to cut up the genetic material within the cell, completing the cell death module.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[http://www.neb.com/nebecomm/products/productR0543.asp Information from NEB]