Difference between revisions of "Part:BBa K200009:Design"

 
(Design Notes)
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<partinfo>BBa_K200009 short</partinfo>
 
<partinfo>BBa_K200009 short</partinfo>
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===Design Notes===
 
===Design Notes===
Dam Sensitivity
 
 
  
 +
NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.
  
 
===Source===
 
===Source===

Revision as of 12:33, 28 August 2009

DpnII Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: Unlike other restriction enzymes that show Star Activity at high pH 8.0, DpnII has Star Activity above approximately pH 6.5. The effect of pH on Star Activity of DpnII is very extreme, showing one-thousand-fold more Star Activity at pH 7.5 than at pH 6.0.

Source

Ecoli

References