Difference between revisions of "Part:BBa K4268000"

Revision as of 14:17, 30 September 2022

S-TIP37 Head-Tail Connector

Usage and Biology

S-TIP37 is a T7-like cyanophage that infects its host via a lytic life cycle [citation]. T7-like phages are characterized by a complex symmetrical capsid structure, which includes an icosahedral head that houses the phage's genome, and an internal core region that stabilizes the packaged DNA inside the head. A neck region that facilitates DNA delivery into a host, and six tail fibers used for attachment to its host.

Figure 1: A labeled visual detailing the various structures of a T7-like phage

This part belongs to a collection that codes for a "ghost" phage. This ghost phage is a capsid-only, empty viral shell that could be modified to immobilize Cyanobacteria recognized by the viral tail fibers, or used with modification to deliver substances to a chassis Cyanobacteria.

However, it will infect Synechococcus sp WH 8109, the cyanobacteria strain that is the natural host of S-TIP37. Further modeling will be needed to determine if the "ghost" phage could effectively target other strains of Cyanobacteria that are used in synthetic biology, such as Synechococcus sp PCC 11901.

The Head-Tail Connector gene (gp25) has been provisionally identified as coding for the neck region of a T7-like phage S-TIP37. The neck region in other T7 viruses serves two main purposes, binding the icosahedral capsid to the tail-like fibers and containing the molecular machinery that is responsible for injecting its contents into the target cyanobacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 346
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-S-TIP 37 is a T7-like cyanophage that virally infects its host via a lytic life cycle. T7-like phages are characterized by a complex symmetrical structure whose major structures are an icosahedral capsid (head) that houses the phage's virus, six tail-like fibers responsible for attachment to its host, and a "neck" region.
+S-TIP37 is a T7-like cyanophage that infects its host via a lytic life cycle [citation]. T7-like phages are characterized by a complex symmetrical capsid structure, which includes an icosahedral head that houses the phage's genome, and an internal core region that stabilizes the packaged DNA inside the head. A neck region that facilitates DNA delivery into a host, and six tail fibers used for attachment to its host.
-[[File:t-suny-oneonta-t7-virus-structure.png|500px|thumb|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage]]
+[[File:T--Suny Oneonta-t7-virus-structure.jpg|200px|frame|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage]]
+This part belongs to a collection that codes for a "ghost" phage. This ghost phage is a capsid-only, empty viral shell that could be modified to immobilize Cyanobacteria recognized by the viral tail fibers, or used with modification to deliver substances to a chassis Cyanobacteria.
-This part belongs to a collection that codes for a "ghost" phage shell that can be used to immobilize or deliver substances, to emerging chassis in synthetic biology. It is unclear if the "ghost" phage will be able to infect ''Synechococcus sp PCC 11901'', a common cyanobacterial engineering chassis. However, it will infect ''Synechococcus sp WH 8109'', the cyanobacteria that S-TIP37 infects. Further modeling will be needed to determine if the "ghost" phage could effectively be used in cyanobacterial engineering.
+However, it will infect ''Synechococcus sp WH 8109'', the cyanobacteria strain that is the natural host of S-TIP37. Further modeling will be needed to determine if the "ghost" phage could effectively target other strains of Cyanobacteria that are used in synthetic biology, such as ''Synechococcus sp PCC 11901''.
 ----
-The Head-Tail Connector codes for the "neck" region of a T7-like phage. The "neck" serves two main purposes, binding the icosahedral capsid to the tail-like fibers and containing the molecular machinery that is responsible for injecting its contents into the target cyanobacteria.
+The Head-Tail Connector gene (gp25) has been provisionally identified as coding for the neck region of a T7-like phage S-TIP37. The neck region in other T7 viruses serves two main purposes, binding the icosahedral capsid to the tail-like fibers and containing the molecular machinery that is responsible for injecting its contents into the target cyanobacteria.