Difference between revisions of "Part:BBa K4268000"

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S-TIP 37 is a T7-like cyanophage that virally infects its host via a lytic life cycle. T7-like phages are characterized by a complex symmetrical structure whose major structures are an icosahedral capsid (head) that houses the phage's virus, six tail-like fibers responsible for attachment to its host, and a "neck" region.
 
S-TIP 37 is a T7-like cyanophage that virally infects its host via a lytic life cycle. T7-like phages are characterized by a complex symmetrical structure whose major structures are an icosahedral capsid (head) that houses the phage's virus, six tail-like fibers responsible for attachment to its host, and a "neck" region.
  
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[[Image:t-suny-oneonta-t7-virus-structure.png|500px|thumb|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage]]
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[[File:t-suny-oneonta-t7-virus-structure.png|500px|thumb|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage]]
  
  

Revision as of 13:31, 30 September 2022


S-TIP37 Head-Tail Connector


Usage and Biology

S-TIP 37 is a T7-like cyanophage that virally infects its host via a lytic life cycle. T7-like phages are characterized by a complex symmetrical structure whose major structures are an icosahedral capsid (head) that houses the phage's virus, six tail-like fibers responsible for attachment to its host, and a "neck" region.

File:T-suny-oneonta-t7-virus-structure.png
Figure 1: A labeled visual detailing the various structures of a T7-like phage


This part belongs to a collection that codes for a "ghost" phage shell that can be used to immobilize or deliver substances, to emerging chassis in synthetic biology. It is unclear if the "ghost" phage will be able to infect Synechococcus sp PCC 11901, a common cyanobacterial engineering chassis. However, it will infect Synechococcus sp WH 8109, the cyanobacteria that S-TIP37 infects. Further modeling will be needed to determine if the "ghost" phage could effectively be used in cyanobacterial engineering.

The Head-Tail Connector codes for the "neck" region of a T7-like phage. The "neck" serves two main purposes, binding the icosahedral capsid to the tail-like fibers and containing the molecular machinery that is responsible for injecting its contents into the target cyanobacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]