Difference between revisions of "Part:BBa K4274030:Design"

 
Line 14: Line 14:
 
===Source===
 
===Source===
  
−
BBa_K4274000, BBa_K849001, BBa_K3552015, BBa_B0034, BBa_B0015
+
BBa_K3552015, BBa_B0034, BBa_K4274000, BBa_K849001,BBa_B0015
  
 
===References===
 
===References===

Revision as of 10:36, 30 September 2022


ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1555
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To optimize the production of alpha-santalene, the part collection we built includes: Parts to optimize the activity of farnesyl pyrophosphate synthase, ERG20F96W (Part: BBa_K4274002) and ptac-RiboJ-B0034-ClSS_S533A-B0034-ERG20_F96W-B0015 (Part: BBa_K4274031). Parts to optimize the seceation of santalene synthase, CmR29M2-ClSSS533A (Part: BBa_K4274001) and ptac-RiboJ-B0034-CmR29M2-ClSS_S533A-B0034-ERG20F96W-B0015 (Part: BBa_K4274032). Parts expressing fusion protein ClSS_S533A-FL-ERG20F96W BBa_K4274003 and BBa_K4274033. Parts expressing fusion protein CmR29M2-ClSS_S533A-FL-ERG20_F96W BBa_K4274004 and BBa_K4274034. Our part collection can be used to help and inspire future teams to design and perfect different alpha-santalene production pathways in E. coli.


Source

BBa_K3552015, BBa_B0034, BBa_K4274000, BBa_K849001,BBa_B0015

References