Difference between revisions of "Part:BBa K4194019"
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===Experiment and result=== | ===Experiment and result=== | ||
| + | ==Gas chromatographic analysis of hydrolyzed products== | ||
The BUCT-China team attempted to synthesize PHFA (polyhydroxyfatty acid, a biocompatible polymer material) using an enzyme system containing CoA Ligase.The obtained product was hydrolyzed and then analyzed by gas chromatography, and the result analysis showed that the monomer of the polymer was hydroxy hexadecanoic acid. Therefore, it can be seen that the system carried out hydroxylation reaction and subsequent polymerization reaction on the fatty acid of the substrate 16C as expected, and the system basically met our requirements. | The BUCT-China team attempted to synthesize PHFA (polyhydroxyfatty acid, a biocompatible polymer material) using an enzyme system containing CoA Ligase.The obtained product was hydrolyzed and then analyzed by gas chromatography, and the result analysis showed that the monomer of the polymer was hydroxy hexadecanoic acid. Therefore, it can be seen that the system carried out hydroxylation reaction and subsequent polymerization reaction on the fatty acid of the substrate 16C as expected, and the system basically met our requirements. | ||
[[File:Results of gas chromatographic analysis of product hydrolysis.png|900px|thumb|left|Results of gas chromatographic analysis of product hydrolysis.]] | [[File:Results of gas chromatographic analysis of product hydrolysis.png|900px|thumb|left|Results of gas chromatographic analysis of product hydrolysis.]] | ||
| + | <br style="clear: both" /> | ||
| + | |||
| + | ==NMR hydrogen spectrogram analysis of polymers== | ||
| + | |||
| + | [[File:The above fig. shows the NMR of the simulated dimer product and the below fig. shows the NMR of the product.png|900px|thumb|left|The above fig. shows the NMR of the simulated dimer product and the below fig. shows the NMR of the product.]] | ||
| + | |||
| + | The characteristic peaks determines that the product has dimerization products. | ||
| + | [[File:The characteristic peaks determines that the product has dimerization products..png|900px|thumb|left|]] | ||
| + | [[File:NMR of hydroxyhexadecanoic acid..png|900px|thumb|left|NMR of hydroxyhexadecanoic acid.]] | ||
| + | [[File:NMR of dimeric compounds.png|900px|thumb|left|NMR of dimeric compounds.]] | ||
| + | <br style="clear: both" /> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K4194019 parameters</partinfo> | <partinfo>BBa_K4194019 parameters</partinfo> | ||
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Revision as of 01:27, 30 September 2022
fadD
The gene codes for long chain acyl-CoA synthase(CoA ligase).
Usage and Biology
CoA ligase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 195
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 504
Illegal XhoI site found at 1006 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 408
Illegal AgeI site found at 1033 - 1000COMPATIBLE WITH RFC[1000]
Experiment and result
Gas chromatographic analysis of hydrolyzed products
The BUCT-China team attempted to synthesize PHFA (polyhydroxyfatty acid, a biocompatible polymer material) using an enzyme system containing CoA Ligase.The obtained product was hydrolyzed and then analyzed by gas chromatography, and the result analysis showed that the monomer of the polymer was hydroxy hexadecanoic acid. Therefore, it can be seen that the system carried out hydroxylation reaction and subsequent polymerization reaction on the fatty acid of the substrate 16C as expected, and the system basically met our requirements.
NMR hydrogen spectrogram analysis of polymers
The characteristic peaks determines that the product has dimerization products.
