Difference between revisions of "Part:BBa K190021:Design"
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===Source=== | ===Source=== | ||
| − | The gene was cloned from pET29a-SmtA (kindly provided by Kevin Waldron, Newcastle University), it's origin is from the genome of ''Synechococcus PC'' | + | The gene was cloned from pET29a-SmtA (kindly provided by Kevin Waldron, Newcastle University), it's origin is from the genome of ''Synechococcus PC'' a Cyanobacterium. |
===References=== | ===References=== | ||
*Microbial Metallothioneins, Robinson et al. Adv in MicroPhys, 2001, 44; 183-213 | *Microbial Metallothioneins, Robinson et al. Adv in MicroPhys, 2001, 44; 183-213 | ||
*Cyanobacterial metallothionein gene expressed in Escherichia Metal-binding properties of the expressed protein, Jianguo Shi et al. FEBS letters, June 1992, Volume 303, number 2,3, 159-163. | *Cyanobacterial metallothionein gene expressed in Escherichia Metal-binding properties of the expressed protein, Jianguo Shi et al. FEBS letters, June 1992, Volume 303, number 2,3, 159-163. | ||
Latest revision as of 15:43, 19 August 2009
SmtA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 111
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 111
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 111
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 111
Illegal AgeI site found at 139 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The BioBrick prefix and suffix were added by PCR, also the RBS (B0034) was added.
Source
The gene was cloned from pET29a-SmtA (kindly provided by Kevin Waldron, Newcastle University), it's origin is from the genome of Synechococcus PC a Cyanobacterium.
References
- Microbial Metallothioneins, Robinson et al. Adv in MicroPhys, 2001, 44; 183-213
- Cyanobacterial metallothionein gene expressed in Escherichia Metal-binding properties of the expressed protein, Jianguo Shi et al. FEBS letters, June 1992, Volume 303, number 2,3, 159-163.