Difference between revisions of "Part:BBa K4140011"

(→‎Usage)
(→‎Usage)
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A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI).
 
A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI).
 
==Usage==
 
==Usage==
−
lac promoter contains A CAP binding site and the lac operator sequence lacO,Expression from the lac promoter is activated in the absence of the lac repressor (LacI).
+
We use the lac promoter as a constitutive promoter for the regulation of (Phenylalanine-specific permease)expression even if it is not as powerful as the tac or trc promoters.it still showing significant levels of phenylalanine enough to induce and start our circuit activity by improving the permeability of the membrane to phenylalanine
−
Although the lac promoter is not as potent as the tac or trc promoters, we use it as a constitutive promoter for the regulation of (Phenylalanine-specific permease) expression.
+
  
 
==Literature Characterization==
 
==Literature Characterization==

Revision as of 08:47, 29 September 2022


Lac promoter

Part Description

A CAP binding site and the lac operator sequence lacO are present in this lac promoter. Although the lac promoter is not as potent as the tac or trc promoters, it nevertheless provides for decent quantities of foreign protein production in high copy-number vectors. Expression from the lac promoter is activated in the absence of the lac repressor (LacI).

Usage

We use the lac promoter as a constitutive promoter for the regulation of (Phenylalanine-specific permease)expression even if it is not as powerful as the tac or trc promoters.it still showing significant levels of phenylalanine enough to induce and start our circuit activity by improving the permeability of the membrane to phenylalanine

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.





























References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]