Difference between revisions of "Part:BBa K4194007"

Revision as of 12:10, 24 September 2022 (view source) Registry (Talk | contribs)		Latest revision as of 02:26, 29 September 2022 (view source) HJ (Talk | contribs)
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	A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.		A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.
−	<!-- Add more about the biology of this part here
−	===Usage and Biology===
−	<!-- -->	+	===Usage and Biology===
		+	==ssrA-LVA tag==
		+	[[File:Mechanism of ssrA-LVA tag.png|300px|thumb|left|In E. coli [wild-type (sspB+ clpX+)], the adaptor sspB binds ssrAtagged substrates to ClpXP proteases, resulting in a modest increase in their degradation rate.]]LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.
	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>
	<partinfo>BBa_K4194007 SequenceAndFeatures</partinfo>		<partinfo>BBa_K4194007 SequenceAndFeatures</partinfo>
−	<!-- Uncomment this to enable Functional Parameter display	+
	===Functional Parameters===		===Functional Parameters===
	<partinfo>BBa_K4194007 parameters</partinfo>		<partinfo>BBa_K4194007 parameters</partinfo>
	<!-- -->		<!-- -->
		+	[[File:The bacteria solution is green because of the expression of EGFP..jpg|200px|thumb|left|The bacteria solution is green because of the expression of EGFP.]]

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Latest revision as of 02:26, 29 September 2022

EGFP（+LVA）

a strong reporter. EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Ex:488nm Em :507nm EC :55,900(M-1 cm-1) QY:0.6 Brightness:33.54 pKa:6.0 Maturation:25.0min Lifetime:2.6ns

A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.

Usage and Biology

ssrA-LVA tag

LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.

Sequence and Features

Functional Parameters