Difference between revisions of "Part:BBa K4329003"
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| − | [[ | + | [[Image: Quorum sensing suicidal system.png|center|frame|100px|<b>Fig. 1 The pathway of pT7-dCas9-sgRNA.</b>]]<br><br> |
At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi/dCas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die. | At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi/dCas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die. | ||
Revision as of 00:49, 29 September 2022
Quorum sensing suicidal system-part1
what is it
The quorum sensing suicidal system consists of three parts: LUXR, CRISPRi/dCas9, and sgRNA. The LUXR protein is regulated by the T7 promoter, lac operator, and T7 terminator. CRISPRi/dCas9 is regulated by a quorum sensing promoter and quorum sensing terminator. sgRNA is regulated by the H1 promoter and H1 terminator.
Usage and Biology
At first, if the IPTG is absent, the RNA ligase in the system will converge with the T7 promoter which can not lead to the production of mRNA. Thus, protein and enzyme production will be inhibited. However, in severe situations, IPTG could be present in the natural environment, so we have considered a double safety system. If LUXR detects the presence of AHL, a quorum sensing chemical sent out by E.Coli, quorum sensing promoter will be activated. Later, the promoter will activate the CRISPRi/dCas9 system targeting the ATP ligase of E.coli. Consequently, without ATP, the bacteria will be forced to die.
Characterization
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]