Difference between revisions of "Part:BBa K4329001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | [[Image: Dethiobiotin.png| | + | [[Image: Dethiobiotin expression.png|100px|frame|center|<b>Fig. 1 The pathway of increasing dethiobiotin synthase and biotin synthase.</b>]]<br><br> |
With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin. | With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin. | ||
Revision as of 00:31, 29 September 2022
Overexpression of dethiobiotin synthase and biotin synthase in E. coli
What is it?
In order to alter the metabolic pathway of E. coli and tend to a more productive way of biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of dethiobiotin synthase and biotin synthase. This plasmid is regulated by the T7 promoter, Lac operator, and T7 terminator.
Usage and Biology
Fig. 1 The pathway of increasing dethiobiotin synthase and biotin synthase.
With IPTG, RNA ligase will converge with the T7 promoter, leading to mRNA production. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating an ample amount of biotin.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]