Difference between revisions of "Part:BBa K4387000"
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− | PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and | + | PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and NorR, the corresponding transcription factor. IHF was removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription. |
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Revision as of 19:43, 28 September 2022
Nitric Oxide Sensing Promoter pNorVβ
PnorV_beta is an optimized nitric oxide sensitive promoter. The promoter is coupled to a positive feedback loop with sfGFP and NorR, the corresponding transcription factor. IHF was removed from the promoter PnorV to exhibit good sensitivity and dosage response at a low range of inducer DETA/NO, the used NO source in the experiment, activating the downstream genes' transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]