Difference between revisions of "Part:BBa K4140020"
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Our aptamer is a single strand DNA with defined and stable tertiary structures that bind to target molecules (phenylalanine) with high affinity and specificity in physiological buffers and fluids and complex biological matrices. Aptamers have made it possible to detect small molecules, including neutral targets, in these environments in sensitive and selective ways | Our aptamer is a single strand DNA with defined and stable tertiary structures that bind to target molecules (phenylalanine) with high affinity and specificity in physiological buffers and fluids and complex biological matrices. Aptamers have made it possible to detect small molecules, including neutral targets, in these environments in sensitive and selective ways | ||
| + | ==Usage== | ||
| + | Our aptamer is a ssDNA that has a very high recognition specificity,affinity and stable tertiary structures that binds to target molecules (phenylalanine) we used aptamers in our LFA to consume the maximum normal level of phenylalanine in the blood leaving the excess phenylalanine to flow the next line test which contains E-coli with a reporter gene in response to phenylalanine that is above normal level. | ||
| + | ==Literature Characterization== | ||
| + | [[File:T--AFCM-EGYPT--CAS2.PNG|thumb|Right|Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs. ]] | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
| + | |||
| + | |||
| + | ==References== | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:15, 28 September 2022
Phenylalanine aptamer 3
Our aptamer is a single strand DNA with defined and stable tertiary structures that bind to target molecules (phenylalanine) with high affinity and specificity in physiological buffers and fluids and complex biological matrices. Aptamers have made it possible to detect small molecules, including neutral targets, in these environments in sensitive and selective ways
Usage
Our aptamer is a ssDNA that has a very high recognition specificity,affinity and stable tertiary structures that binds to target molecules (phenylalanine) we used aptamers in our LFA to consume the maximum normal level of phenylalanine in the blood leaving the excess phenylalanine to flow the next line test which contains E-coli with a reporter gene in response to phenylalanine that is above normal level.
Literature Characterization
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]