Difference between revisions of "Part:BBa K3743020"
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It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle | It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle | ||
==Usage== | ==Usage== | ||
| − | + | It is the RNA domain of the CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle we use this part to guide Cas12g protein to the complimentary site on mRNA of PAH to inhibit it’s expression when phenylalanine level is normal or to prevent over expression of PAH. | |
| − | + | ||
==Literature Characterization== | ==Literature Characterization== | ||
Revision as of 17:58, 28 September 2022
VSVg-Fusogen
Part Description
It is the RNA domain of CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle
Usage
It is the RNA domain of the CRISPR system which formed of two component crRNA that bind to the target sequence and tracrRNA that connect cas protein to the RNA which act as a handle we use this part to guide Cas12g protein to the complimentary site on mRNA of PAH to inhibit it’s expression when phenylalanine level is normal or to prevent over expression of PAH.
Literature Characterization
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1396
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1176
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 987
- 1000COMPATIBLE WITH RFC[1000]