Difference between revisions of "Part:BBa K4152002"

 
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<partinfo>BBa_K4152002 short</partinfo>
 
<partinfo>BBa_K4152002 short</partinfo>
  
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To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT7 is a complicated mutation of Proteinase K, which contains 7 mutation sites: D260N-N257C-P18Q-S173W-S197F-T16C-T20D.
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To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT2 is a complicated mutation of Proteinase K, which contains 7 mutation sites: D260N-N257C-P18Q-S173W-S197F-T16C-T20D.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:41, 28 September 2022


PK_MT2

To improve the performance of Proteinase K, we designed many Proteinase K mutant genes. PK_MT2 is a complicated mutation of Proteinase K, which contains 7 mutation sites: D260N-N257C-P18Q-S173W-S197F-T16C-T20D.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 235
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 235
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]