Difference between revisions of "Part:BBa K4447001:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4447001 short</partinfo>
 
<partinfo>BBa_K4447001 short</partinfo>
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<div style="text-align:justify;">
 
<partinfo>BBa_K4447001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4447001 SequenceAndFeatures</partinfo>
  
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===Design Notes===
 
===Design Notes===
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
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===Source===
 
===Source===
  
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The original sequence was reported by Stassi et al. (1993). Sequence can be obtained through GenBank (L05776).
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The original sequence was reported by Stassi <i>et al.</i> (1993). Sequence can be obtained through GenBank (L05776).
  
 
===References===
 
===References===
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[1]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a <i>Saccharopolyspora erythraea</i> gene required for the final hydroxylation step in erythromycin biosynthesis. <i>Journal of bacteriology, 175</i>(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993

Latest revision as of 16:45, 28 September 2022


EryK coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.

Source

The original sequence was reported by Stassi et al. (1993). Sequence can be obtained through GenBank (L05776).

References

[1]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. Journal of bacteriology, 175(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993