Difference between revisions of "Part:BBa K4447002:Design"
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===Design Notes===
 
===Design Notes===
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
 
A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.
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===Source===
 
===Source===
  
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The original sequence was reported by Orser et al. (1993). Sequence can be obtained through GenBank (M98557.1).
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The original sequence was reported by Orser <i>et al.</i> (1993). Sequence can be obtained through GenBank (M98557.1).
  
 
===References===
 
===References===
  
 
[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in <i>Escherichia coli</i>. <i>Journal of bacteriology, 175</i>(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993
 
[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in <i>Escherichia coli</i>. <i>Journal of bacteriology, 175</i>(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993

Latest revision as of 16:38, 28 September 2022


Pcp coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

A gly-gly-ser spacer, and a polyhistidine-tag were added before stop codon for protein purification. NcoI and XhoI restriction sites were added in 5' and 3' ends for protein overexpression in pBAD/Myc-His plasmids.

Source

The original sequence was reported by Orser et al. (1993). Sequence can be obtained through GenBank (M98557.1).

References

[1]. Orser, C. S., Lange, C. C., Xun, L., Zahrt, T. C., & Schneider, B. J. (1993). Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli. Journal of bacteriology, 175(2), 411–416. https://doi.org/10.1128/jb.175.2.411-416.1993