Difference between revisions of "Part:BBa K4140000"

Revision as of 16:03, 28 September 2022

T7 promoter

Part Description

T7 promoter is a dna sequence that can be recognized by T7 RNA polymerase and is mainly used to regulate gene expression of recombinant proteins,all of which allows it to be used in downstream research approaches.It is considered to be a constitutive promoter with high levels of transcription and expression in the presence of T7 RNAP and minimal interaction with bacterial RNAP.

Usage

Literature Characterization

Figure 1.Substrate RNA cleavage assay using wild-type and mutant target RNAs.

References

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ==Part Description==
-Constitutive promoter with high levels of transcription and expression in the presence of T7 RNAP,with minimal interaction with bacterial RNAP
+T7 promoter is a dna sequence that can be recognized by T7 RNA polymerase and is mainly used to regulate gene expression of recombinant proteins,all of which allows it to be used in downstream research approaches.It is considered to be a  constitutive promoter with high levels of transcription and expression in the presence of T7 RNAP and minimal interaction with bacterial RNAP.
 ==Usage==