Difference between revisions of "Part:BBa K4329004"

 
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By using CRISPR i system, we are able to decrease the expression of Bifunctional BirA enzyme which is an enzyme to consume the biotin in the E. coli. In our project, we are aiming at biosynthesizing biotin through the metabolic pathways to generate more biotin in the E. coli.   
 
By using CRISPR i system, we are able to decrease the expression of Bifunctional BirA enzyme which is an enzyme to consume the biotin in the E. coli. In our project, we are aiming at biosynthesizing biotin through the metabolic pathways to generate more biotin in the E. coli.   
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===Usage and Biology===
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===Characterization===
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Revision as of 12:07, 28 September 2022


Knockdown of Bifunctional BirA enzyme

By using CRISPR i system, we are able to decrease the expression of Bifunctional BirA enzyme which is an enzyme to consume the biotin in the E. coli. In our project, we are aiming at biosynthesizing biotin through the metabolic pathways to generate more biotin in the E. coli.

Usage and Biology

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1202
    Illegal NheI site found at 4217
    Illegal NheI site found at 4240
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49