Difference between revisions of "Part:BBa K4232002"
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The nirB activity is affected by nitrate and its use needs to be considered with or without removing this effect. | The nirB activity is affected by nitrate and its use needs to be considered with or without removing this effect. | ||
| − | [[File:BIT-China-parts-nirb-pcr.jpeg|350px|left|'''Figure 1'''Results of PCR about nirb]] | + | [[File:BIT-China-parts-nirb-pcr.jpeg|350px|thumb|left|'''Figure 1'''Results of PCR about nirb]] |
[[File:BIT-China-parts-nirb-expression.jpeg|400px|right|'''Figure 2'''Expression of nirb at different oxygen concentrations]] | [[File:BIT-China-parts-nirb-expression.jpeg|400px|right|'''Figure 2'''Expression of nirb at different oxygen concentrations]] | ||
Revision as of 02:33, 28 September 2022
A oxygen-sensitive promoter
This part codes for the promoter nirB. The nirB promoter, known as nitrite reductase (NADH) large subunit, was derived from the E. coli top10 strain (PCR image shown in Figure 1). It can be used as a sensor of intracellular oxygen concentration due to the fact that it is expressed at much higher levels under low oxygen concentration conditions than under normal oxygen concentration (as shown in Figure 2), regardless of the presence of nitrite or nitrate in the environment
The nirB activity is affected by nitrate and its use needs to be considered with or without removing this effect.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 409
Illegal BglII site found at 1278 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1849
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2396
Illegal SapI.rc site found at 68
Illegal SapI.rc site found at 2351