Difference between revisions of "Part:BBa K196005:Design"

 
 
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<partinfo>BBa_K196005 short</partinfo>
 
<partinfo>BBa_K196005 short</partinfo>
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===Design Notes===
 
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===Source===
  
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HfsG and HfsH come from Caulobacter crescentus but were synthesized by Geneart [http://www.geneart.com] to satisfy de standard 10.
  
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===Source===
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===References===
  
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HfsG and HfsH come from Caulobacter crescentus but were synthesized by Geneart to satisfy de standard 10.
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Toh, E, H. D. Kurtz, Jr., and Y.V. Brun. 2008. '''Characterization of the Caulobacter crescentus Holdfast Polysaccharide Biosynthesis Pathway Reveals Significant Redundancy in the Initiating Glycosyltransferase and Polymerase steps'''. ''Journal of Bacteriology'', 190, 7219-7231.
  
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===References===
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Smith, C. S., A. Hinz, D. Bodenmiller, D. E. Larson, and Y. V. Brun. 2003. '''Identification of genes required for synthesis of the adhesive holdfast in Caulobacter crescentus.''' ''J. Bacteriol''. 185:1342-1442.

Latest revision as of 10:03, 13 August 2009

HfsG + HfsH proteins from Caulobacter crescentus + ter (BBa_B0015)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal AgeI site found at 379
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

HfsG and HfsH come from Caulobacter crescentus but were synthesized by Geneart [http://www.geneart.com] to satisfy de standard 10.

References

Toh, E, H. D. Kurtz, Jr., and Y.V. Brun. 2008. Characterization of the Caulobacter crescentus Holdfast Polysaccharide Biosynthesis Pathway Reveals Significant Redundancy in the Initiating Glycosyltransferase and Polymerase steps. Journal of Bacteriology, 190, 7219-7231.

Smith, C. S., A. Hinz, D. Bodenmiller, D. E. Larson, and Y. V. Brun. 2003. Identification of genes required for synthesis of the adhesive holdfast in Caulobacter crescentus. J. Bacteriol. 185:1342-1442.