Difference between revisions of "Part:BBa K4409004"

 
 
(2 intermediate revisions by 2 users not shown)
Line 1: Line 1:
  
 +
__NOTOC__
 +
<partinfo>BBa_K4409004 short</partinfo>
 +
 +
It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (GAPDH) (BBa_K4409017). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (GAPDH) (BBa_K4409017) which can then bind with dsDNA (GAPDH) (BBa_K4409006) and activate Cas enzyme to cleave DNAs.
 +
 +
<div style='text-align: center;'>
 +
 +
[[File:Hz1-part-1-3.png|600px|thumb|center]]
 +
 +
<b> 【Graph 1】The made up of igRNA </b>
 +
</div>
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K4409004 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K4409004 parameters</partinfo>
 +
<!-- -->

Latest revision as of 08:06, 27 September 2022


trigger-RNA sensor of igRNA (hsa_circ_0001982)_Coupled with GAPDH as guide sequence

It is used as a sensing part of the RNA-interacting guide RNA (igRNA) in our CRISPR-Cas12 system to detect a unique sequence (trigger RNA) of the target circRNA hsa_circ_0001982. This part serves as a switch for the whole circuit. It contains three regions, VR1 region, loop region, and VR2 region. VR1 and VR2 regions complement to VR1’ and VR2’ regions (trigger RNA) of hsa_circ_0001982. Loop region is complementary to the guide of igRNA (GAPDH) (BBa_K4409017). When hsa_circ_0001982 is present in the system, VR1 and VR2 regions combine with VR1’ and VR2’ sequences. Thus, loop region unblocks the guide of igRNA (GAPDH) (BBa_K4409017) which can then bind with dsDNA (GAPDH) (BBa_K4409006) and activate Cas enzyme to cleave DNAs.

Hz1-part-1-3.png

【Graph 1】The made up of igRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]