Difference between revisions of "Part:BBa K4409004:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K4409004 short</partinfo> | ||
+ | |||
+ | <partinfo>BBa_K4409004 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | ===Design Notes=== | ||
+ | |||
+ | The sequence is comprised of three regions, VR1 region, loop region and VR2 region. VR1 region should complement to VR1’ region of the target circRNA hsa_circ_0001982. Loop region should complement to the guide of igRNA (GAPDH) (BBa_K4409017). VR2 region should complement to VR2’ region of hsa_circ_0001982. VR1’ and VR2’ regions should cover a unique sequence of hsa_circ_0001982 so that VR1 and VR2 regions can specifically recognize hsa_circ_0001982. | ||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | VR1 and VR2 regions of this part are based on the complement sequence of hsa_circ_0001982 sequence which is retrieved from circBank database. | ||
+ | Loop region is based on BBa_K4409017. | ||
+ | |||
+ | ===References=== |
Latest revision as of 07:26, 27 September 2022
trigger-RNA sensor of igRNA (hsa_circ_0001982)_Coupled with GAPDH as guide sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is comprised of three regions, VR1 region, loop region and VR2 region. VR1 region should complement to VR1’ region of the target circRNA hsa_circ_0001982. Loop region should complement to the guide of igRNA (GAPDH) (BBa_K4409017). VR2 region should complement to VR2’ region of hsa_circ_0001982. VR1’ and VR2’ regions should cover a unique sequence of hsa_circ_0001982 so that VR1 and VR2 regions can specifically recognize hsa_circ_0001982.
Source
VR1 and VR2 regions of this part are based on the complement sequence of hsa_circ_0001982 sequence which is retrieved from circBank database. Loop region is based on BBa_K4409017.