Difference between revisions of "Part:BBa K4046050"
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This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.  
 
This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.  
  
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===Design Notes===
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===Part Contributions===
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The start codon ATG is not included in this version of the Kozak sequence.
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'''Group:''' iGEM22_Stony_Brook
  
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===Source===
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'''Authors:''' Maulik Masaliya
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The Kozak sequence was determined through analysis of various eukaryotic mRNAs.
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'''Contribution Summary'''<br>
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This is the vertebrate consensus Kozak sequence, added upstream of the start codon of our insert
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<partinfo>BBa_K4235000</partinfo>. The Kozak sequence serves as a translation initiation site in eukaryotes and ensures proper translation the protein from the mRNA transcript.
  
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===References===
 
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Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349.
 
  
 
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Latest revision as of 01:17, 27 September 2022


Kozak Sequence (Human RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.

Part Contributions

Group: iGEM22_Stony_Brook

Authors: Maulik Masaliya

Contribution Summary
This is the vertebrate consensus Kozak sequence, added upstream of the start codon of our insert BBa_K4235000. The Kozak sequence serves as a translation initiation site in eukaryotes and ensures proper translation the protein from the mRNA transcript.