Difference between revisions of "Part:BBa K3814029"
| Line 10: | Line 10: | ||
'''Authors:''' Maulik Masaliya | '''Authors:''' Maulik Masaliya | ||
| − | '''Contribution Summary''' | + | '''Contribution Summary'''<br> |
The pC promoter was one the parts we intended on using for our Sf9 baculovirus expression system. | The pC promoter was one the parts we intended on using for our Sf9 baculovirus expression system. | ||
This promoter drives expression of the Gentamicin resistance gene and is part of the entire Gentamicin Resistance gene cassette which is used within the plasmid <partinfo>BBa_K4235002</partinfo>. | This promoter drives expression of the Gentamicin resistance gene and is part of the entire Gentamicin Resistance gene cassette which is used within the plasmid <partinfo>BBa_K4235002</partinfo>. | ||
Latest revision as of 00:48, 27 September 2022
Pc promoter
Promoter used to express the GmR selectable marker gene.
Part Contributions
Group: iGEM22_Stony_Brook
Authors: Maulik Masaliya
Contribution Summary
The pC promoter was one the parts we intended on using for our Sf9 baculovirus expression system.
This promoter drives expression of the Gentamicin resistance gene and is part of the entire Gentamicin Resistance gene cassette which is used within the plasmid BBa_K4235002.
This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]