Difference between revisions of "Part:BBa K3814029"

 
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Promoter used to express the GmR selectable marker gene.
 
Promoter used to express the GmR selectable marker gene.
  
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===Part Contributions===
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'''Group:''' iGEM22_Stony_Brook
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'''Authors:''' Maulik Masaliya
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'''Contribution Summary'''
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The pC promoter was one the parts we intended on using for our Sf9 baculovirus expression system.
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This promoter drives expression of the Gentamicin resistance gene and is part of the entire Gentamicin Resistance gene cassette which is used within the plasmid <partinfo>BBa_K4235002</partinfo>.
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This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.
  
 
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Revision as of 00:42, 27 September 2022


Pc promoter

Promoter used to express the GmR selectable marker gene.

Part Contributions

Group: iGEM22_Stony_Brook

Authors: Maulik Masaliya

Contribution Summary The pC promoter was one the parts we intended on using for our Sf9 baculovirus expression system. This promoter drives expression of the Gentamicin resistance gene and is part of the entire Gentamicin Resistance gene cassette which is used within the plasmid BBa_K4235002.

This marker is used to provide gentamicin resistance to the pFastBac vector during the transposition in the Bacmid, propagated in E coli DH10Bac cells. A concentration of 7 ug/ml gentamicin is recommended for transposition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]