Difference between revisions of "Part:BBa K176042:Design"

 
 
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<partinfo>BBa_K176042 short</partinfo>
 
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Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
 
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.
  
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Main parameters for picking VR3~VR12:
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Size: 20 bp (N19 + G or C)
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Tm: VF2 Tm ±1℃
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GC%: 50%
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Max Self Complementarity: 3 bp
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Max 3' Self Complementarity: 2 bp
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Max Pair Complementarity: 3 bp
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Max 3' Pair Complementarity: 2 bp
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Max 3' Stability: 3.36 kcal/mol
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Max Template(pSB1A3) Mispriming: 6 bp
  
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Max Mispriming With Other VR3~VR12 Sites: 4 bp
  
 
===Source===
 
===Source===

Latest revision as of 07:49, 13 August 2009

Reverse primer VR8 (K176052) binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Main parameters for picking VR3~VR12:

Size: 20 bp (N19 + G or C)

Tm: VF2 Tm ±1℃

GC%: 50%

Max Self Complementarity: 3 bp

Max 3' Self Complementarity: 2 bp

Max Pair Complementarity: 3 bp

Max 3' Pair Complementarity: 2 bp

Max 3' Stability: 3.36 kcal/mol

Max Template(pSB1A3) Mispriming: 6 bp

Max Mispriming With Other VR3~VR12 Sites: 4 bp

Source

Reverse complement of VR8 (K176052). Picked from random sequences.

References