Difference between revisions of "Part:BBa K4257000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Highly active version of E.coli native | + | Highly active version of E. coli native PPK. |
===Source=== | ===Source=== | ||
Latest revision as of 16:18, 26 September 2022
PPK-M
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Highly active version of E. coli native PPK.
Source
The PPK-M coding sequence was obtained by PCR-mediated site-directed mutagenesis using the genomic DNA of E.coli K12 as the template.
References
Rudat, A. K., et al. (2018). "Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels." 200(6): e00697-00617.