Difference between revisions of "Part:BBa K4220011"
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<partinfo>BBa_K4220011 short</partinfo> | <partinfo>BBa_K4220011 short</partinfo> | ||
− | + | ==Introduction:== | |
+ | |||
+ | RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium. | ||
+ | |||
+ | ==Composition: == | ||
+ | |||
+ | 1.Functional structure: ZNRF3+linker+LGR4+human IgGH3 Fc | ||
+ | |||
+ | 2.alpha factor sp: the signaling peptide of alpha factor in the yeast mating pathway | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 13:33, 26 September 2022
alpha factor sp-V5-RTAC
Introduction:
RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.
Composition:
1.Functional structure: ZNRF3+linker+LGR4+human IgGH3 Fc
2.alpha factor sp: the signaling peptide of alpha factor in the yeast mating pathway
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 471
Illegal XbaI site found at 1404
Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1407
Illegal BamHI site found at 2288
Illegal XhoI site found at 2279 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 471
Illegal XbaI site found at 1404
Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 471
Illegal XbaI site found at 1404
Illegal PstI site found at 321
Illegal PstI site found at 760
Illegal PstI site found at 2132
Illegal AgeI site found at 2391 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 283
Illegal BsaI site found at 1604
Illegal BsaI.rc site found at 1292