Difference between revisions of "Part:BBa K4226000"
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==Experimental Setup== | ==Experimental Setup== | ||
| + | *We designed the genetic circuit that contains sulAp, Amp30E, relE gene, mScarlet-I and 3WJ-Bro, and transferred it into DH10B competent cells | ||
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=== Characterized by CAFA_China 2022 === | === Characterized by CAFA_China 2022 === | ||
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[[File:3WJ-Bro.png|600px|thumb|center|3WJ-Bro was ligated with fluorescent aptamers in order to examine the level of relE RNA synthesis (485/510nm).]]<br> | [[File:3WJ-Bro.png|600px|thumb|center|3WJ-Bro was ligated with fluorescent aptamers in order to examine the level of relE RNA synthesis (485/510nm).]]<br> | ||
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| + | <!-- --> | ||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K4226000 SequenceAndFeatures</partinfo> | ||
Revision as of 06:19, 26 September 2022
3WJ-Bro
3WJ-Bro is used as a protein- independent reporter system based on a fluorescent RNA aptamer. It is applied as a gene marker in creating a system for reporting the presence and expression of target genes. The 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level in Escherichia coli cells, obviating the need for accumulation of foreign proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]