Difference between revisions of "Part:BBa K302018:Experience"
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| + | ==User Reviews== | ||
| + | Liaoliao_Gao<br> | ||
| + | In 2022, ZJU-China iGEM team has characterized the output of this part in a novel chassis E. coli BL21(DE3). The result was documented in the experience page and the main page of (<partinfo>BBa_K302018</partinfo>).<br> | ||
| + | ===Contribution from iGEM2022 ZJU-China=== | ||
| + | <p>The sequence of (<partinfo>BBa_K302018</partinfo>) was synthesized and cloned into the PHY-plasmid to obtain the recombinant expression vector </p> | ||
| + | <p>We first tested the inhibitory concentration of subtilisin by <b>zone of inhibition experiment</b>, using BL21 transformed by PHY-Immunity plasmid or PHY-Empty plasmid as control. The concentration of subtilisin were 100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml. </p> | ||
| + | <div align="center">[[File:GLL-2.png|600px]]</div> | ||
| + | <center>Fig1 Zone of inhibition experiment for subtilisin in BL21. | ||
| + | </center> | ||
| + | <!-- --> | ||
Revision as of 03:45, 26 September 2022
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K302018
User Reviews
UNIQ1edae86fb37df900-partinfo-00000000-QINU UNIQ1edae86fb37df900-partinfo-00000001-QINU
User Reviews
Liaoliao_Gao
In 2022, ZJU-China iGEM team has characterized the output of this part in a novel chassis E. coli BL21(DE3). The result was documented in the experience page and the main page of (BBa_K302018).
Contribution from iGEM2022 ZJU-China
The sequence of (BBa_K302018) was synthesized and cloned into the PHY-plasmid to obtain the recombinant expression vector
We first tested the inhibitory concentration of subtilisin by zone of inhibition experiment, using BL21 transformed by PHY-Immunity plasmid or PHY-Empty plasmid as control. The concentration of subtilisin were 100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml.
