Difference between revisions of "Part:BBa K4223012"
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<partinfo>BBa_K4223012 short</partinfo> | <partinfo>BBa_K4223012 short</partinfo> | ||
| − | 5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3' | + | 5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'<br> |
| − | crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the <partinfo>K4223001</partinfo>,<partinfo>K4223009</partinfo>. | + | crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the transcription products of <partinfo>K4223001</partinfo>,<partinfo>K4223009</partinfo> for false positive test.<br> |
| + | [[File:DNA template of crRNA for Cas13a1 in vitro detection system.png|800px|]]<br> | ||
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Latest revision as of 17:00, 25 September 2022
DNA template of crRNA for Cas13a1 in vitro detection system
5'-UAGACCACCCCAAAAAUGAAGGGGACUAAAACAACUAUACAACCUACUACCUCA-3'
crRNA are small guide RNAs that direct the RNAse activity of the Cas13a1 protein against the RNA they hybridize to. Here the crRNA is designed to hybridize to the transcription products of BBa_K4223001,BBa_K4223009 for false positive test.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]