Difference between revisions of "Part:BBa K4202004"

Revision as of 11:33, 25 September 2022

TSLV-BS-CA

Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, α-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO₂ to rapidly produce bicarbonate (HCO₃^-) and protons (H⁺).
Chemical reaction equation: H₂O+CO₂↔HCO₃^-+H⁺
Bicarbonate (HCO₃^-) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca²⁺) in the extracellular medium, the bicarbonate can combine to the Ca²⁺ to form calcium carbonate precipitates. The calcium carbonate precipitate can accumulate in the tiny cracks of the stone artifacts, filling the cracks and providing support.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 540
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 166
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 535

@@ Line 5: / Line 5: @@
 Biomineralization can be used to deposit calcium carbonate on the surface of microbial cells, filling cracks in stone artifacts. This part is the coding sequence (CDS) of Carbonic anhydrase (CA) encoding a zinc-containing enzyme, &#945;-carbonic anhydrase, which efficiently catalyzes the reversible hydration of CO<sub>2</sub> to rapidly produce bicarbonate (HCO<sub>3</sub><sup>-</sup>) and protons (H<sup>+</sup>).
 <br>
-Chemical reaction equations: H<sub>2</sub>O+CO<sub>2</sub>&#8596;HCO<sub>3</sub><sup>-</sup>+H<sup>+</sup>
+Chemical reaction equation: H<sub>2</sub>O+CO<sub>2</sub>&#8596;HCO<sub>3</sub><sup>-</sup>+H<sup>+</sup>
 <br>
 Bicarbonate (HCO<sub>3</sub><sup>-</sup>) can be transported down the concentration gradient to the outside of the cell. When we provide calcium ions (Ca<sup>2+</sup>) in the extracellular medium, the bicarbonate can combine to the Ca<sup>2+</sup> to form calcium carbonate precipitates. The calcium carbonate precipitate can accumulate in the tiny cracks of the stone artifacts, filling the cracks and providing support.