Difference between revisions of "Part:BBa K4329002"
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<partinfo>BBa_K4329002 short</partinfo> | <partinfo>BBa_K4329002 short</partinfo> | ||
| − | + | ===what is it?=== | |
| + | |||
| + | CRISPR/Cas9 is a gene editing technology used to arbitrarily removes 2-7 genes from the targeted sequence inducing DNA to activate its self-repair process. The newly formed DNA will contain incorrect DNA sequences that could not be translated thereby inhibiting the function of the specific DNA chain. | ||
| + | |||
| + | ===What it does?=== | ||
| + | |||
| + | This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGYYAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration. | ||
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Revision as of 16:03, 24 September 2022
sgRNA for targeting Bifunctional BirA enzyme
what is it?
CRISPR/Cas9 is a gene editing technology used to arbitrarily removes 2-7 genes from the targeted sequence inducing DNA to activate its self-repair process. The newly formed DNA will contain incorrect DNA sequences that could not be translated thereby inhibiting the function of the specific DNA chain.
What it does?
This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGYYAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 19
- 1000COMPATIBLE WITH RFC[1000]