Difference between revisions of "Part:BBa K4329001"
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| + | This plasmid is regulated by T7 promoter, Lac operator, and T7 terminator. With IPTG, RNA ligase will converge with T7 promoter which will then lead to the production of mRNA. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating large amount of biotin. | ||
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Revision as of 16:02, 24 September 2022
Overexpression of dethiobiotin synthase and biotin synthase in E. coli
what is it?
This plasmid is regulated by T7 promoter, Lac operator, and T7 terminator. With IPTG, RNA ligase will converge with T7 promoter which will then lead to the production of mRNA. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating large amount of biotin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]