Difference between revisions of "Part:BBa K4329000"
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| − | + | This part includes four substance: TurboID, AIP, biotin,and N-AgrD. TurboID, a pair of biotin ligases, was designed to overcome the problem of slow existing biotin ligases. Using yeast display directed evolution techniques, the labeling time will be shorted to less than 10 minutes. This enables probing of dynamic biological processes with higher temporal resolution. AIP, autoinducing peptide, is a chemical released by S.aureus as it activates quorum sensing. N-AgrD is also a chemical involved in the cycle of virulence production, and it will direct TurboID toward the target site, AgrB. | |
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Revision as of 15:55, 24 September 2022
TurboID-N_AgrD-AIP
What is it?
This part includes four substance: TurboID, AIP, biotin,and N-AgrD. TurboID, a pair of biotin ligases, was designed to overcome the problem of slow existing biotin ligases. Using yeast display directed evolution techniques, the labeling time will be shorted to less than 10 minutes. This enables probing of dynamic biological processes with higher temporal resolution. AIP, autoinducing peptide, is a chemical released by S.aureus as it activates quorum sensing. N-AgrD is also a chemical involved in the cycle of virulence production, and it will direct TurboID toward the target site, AgrB.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]