Difference between revisions of "Part:BBa K4370012"

 
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<partinfo>BBa_K4370012 short</partinfo>
 
<partinfo>BBa_K4370012 short</partinfo>
  
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This part encodes a single guide RNA targeting the <i> gpm </i> gene encoding a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (short name: phosphoglyceromutase) in <i> Streptomyces </i>. It has been designed to target different strains such as <i> S. ambofaciens </i> ATCC 23877, <i> S. bottropensis </i> ATCC 25435, and <i> S. venezuelae </i> ATCC 10712.
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This part encodes a single guide RNA targeting the <i> gpm </i> gene encoding a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (short name: phosphoglyceromutase) in <i> Streptomyces</i>. It has been designed to target different strains such as <i> S. ambofaciens </i> ATCC 23877, <i> S. bottropensis </i> ATCC 25435, and <i> S. venezuelae </i> ATCC 10712.
  
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A decrease of <i> gpm </i> expression has been shown as required to allow the implementation of the Calcin cycle in <i> E. coli </i> (Antonovsky et al., Cell, 2016 - http://dx.doi.org/10.1016/j.cell.2016.05.064). This part could therefore be useful to implement a CO2 fixation module in <i> Streptomyces</i>.
 
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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A decrease of <i> gpm </i> expression has been shown as required to allow the implementation of the Calcin cycle in <i> E. coli </i> (Antonovsky <i>et al., Cell</i>, 2016). This part could therefore be useful to implement a CO<sub>2</sub> fixation module in <i> Streptomyces</i>.
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We have engineered genetic tools via the Crispr-dCas9 system to switch off <i>gpm</i> and force the appearance of the Calvin cycle in their primary metabolism.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4370012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4370012 SequenceAndFeatures</partinfo>
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===References===
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Antonovsky N, Gleizer S, Noor E, Zohar Y, Herz E, Barenholz U, Zelcbuch L, Amram S, Wides A, Tepper N, Davidi D, Bar-On Y, Bareia T, Wernick DG, Shani I, Malitsky S, Jona G, Bar-Even A, Milo R. "Sugar Synthesis from CO<sub>2</sub> in <i>Escherichia coli</i>." Cell. 2016 Jun 30;166(1):115-25
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https://doi.org/10.1016/j.cell.2016.05.064
  
  

Latest revision as of 19:46, 23 September 2022


sgRNA_gpm_STREPTO

This part encodes a single guide RNA targeting the gpm gene encoding a 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (short name: phosphoglyceromutase) in Streptomyces. It has been designed to target different strains such as S. ambofaciens ATCC 23877, S. bottropensis ATCC 25435, and S. venezuelae ATCC 10712.

Usage and Biology

A decrease of gpm expression has been shown as required to allow the implementation of the Calcin cycle in E. coli (Antonovsky et al., Cell, 2016). This part could therefore be useful to implement a CO2 fixation module in Streptomyces.

We have engineered genetic tools via the Crispr-dCas9 system to switch off gpm and force the appearance of the Calvin cycle in their primary metabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Antonovsky N, Gleizer S, Noor E, Zohar Y, Herz E, Barenholz U, Zelcbuch L, Amram S, Wides A, Tepper N, Davidi D, Bar-On Y, Bareia T, Wernick DG, Shani I, Malitsky S, Jona G, Bar-Even A, Milo R. "Sugar Synthesis from CO2 in Escherichia coli." Cell. 2016 Jun 30;166(1):115-25

https://doi.org/10.1016/j.cell.2016.05.064