Difference between revisions of "Part:BBa K4268000:Design"

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To determine which sequences would be used to code for the shell of the phage, a series of comparisons were made. SUNY_Oneonta iGEM had identified T7 and T4-like phages. The team chose to utilize T7-like phages due to their smaller genome compared to T4-like phages. The smaller genome would allow greater efficiency and ease in cloning the chosen species' genes.
 
To determine which sequences would be used to code for the shell of the phage, a series of comparisons were made. SUNY_Oneonta iGEM had identified T7 and T4-like phages. The team chose to utilize T7-like phages due to their smaller genome compared to T4-like phages. The smaller genome would allow greater efficiency and ease in cloning the chosen species' genes.
  
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"Synechococcus sp PCC 11901", was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell.
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''Synechococcus sp PCC 11901'', was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell.
  
  

Revision as of 11:22, 23 September 2022


S-TIP37 Head-Tail Connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To determine which sequences would be used to code for the shell of the phage, a series of comparisons were made. SUNY_Oneonta iGEM had identified T7 and T4-like phages. The team chose to utilize T7-like phages due to their smaller genome compared to T4-like phages. The smaller genome would allow greater efficiency and ease in cloning the chosen species' genes.

Synechococcus sp PCC 11901, was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell.


Insert poster table comparison

--- Upon the isolation of the Head-Tail Connector sequence, the following edits were made:

  • Restriction sites were removed/altered to ensure RCF10 and RCF1000 compatibility
  • To complete Level 0 Golden Gate Assembly, the sequence contains SapI sites at the ends

Source

The source of this sequence is from the S-TIP37 genome (GenBanK: NC_048026.1)

References

The S-TIP37 genome was found on GenomeNet