Difference between revisions of "Part:BBa K4235030"
| Line 7: | Line 7: | ||
[[Image:Ecoli_circuit.png|700px|center|]] | [[Image:Ecoli_circuit.png|700px|center|]] | ||
| + | ===Characterization and results=== | ||
| + | We successfully cloned our insert <partinfo>BBa_K4235011</partinfo> into the pET His6 vector <partinfo>BBa_K4235016</partinfo> using Ligation independent cloning. Please refer the PROS1 insert page <partinfo>BBa_K4235011</partinfo> for results. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 23:42, 22 September 2022
PROS1 expression cassette
This is the entire expression circuit for the Human protein S gene.
Characterization and results
We successfully cloned our insert BBa_K4235011 into the pET His6 vector BBa_K4235016 using Ligation independent cloning. Please refer the PROS1 insert page BBa_K4235011 for results. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1989
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1159
- 1000COMPATIBLE WITH RFC[1000]