Difference between revisions of "Part:BBa K4361315"

 
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<ul>
  
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<li>.</li>
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<li>A 478 > T, resulting in substitution T160S</li>
 +
<li>Insertion of A after A 732, resulting in a possible frameshift and extension of the protein</li>
 +
<li>Insertion of A after A 800, resulting in a possible frameshift and extension of the protein</li>
 
</ul>
 
</ul>
  

Revision as of 14:35, 22 September 2022


BlcR A67H

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.6 A67H (Part:BBa_K4361224). For this mutant, the alanine in position 67 has been changed to histidine by mutating the GCG codon to CAT.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • A 478 > T, resulting in substitution T160S
  • Insertion of A after A 732, resulting in a possible frameshift and extension of the protein
  • Insertion of A after A 800, resulting in a possible frameshift and extension of the protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589